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T0237

100X TE Buffer, pH 8.0. 4L, Sterile, DNase/RNase Tested, PCR Grade.

Availability

Ships in 6 - 14 Days

Container Size

4L

Item Count

1 Bottle(s)

Price per Item

$538.00

Fill Volume

4000 mL

T0237

100X TE Buffer, pH 8.0. 4L, Sterile, DNase/RNase Tested, PCR Grade.

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

100X TE Buffer, pH 8.0, DNase/RNase Tested, PCR Grade is a concentrated Tris-EDTA buffer for nucleic acid workflows, including preparation of working TE solutions for resuspension, dilution, and storage of DNA and RNA. The pH 8.0 buffering system maintains stable conditions during routine molecular biology procedures, and the EDTA component chelates divalent cations such as Mg2+. DNase/RNase testing and PCR-grade specifications support applications that require low nuclease background, including PCR setup and downstream enzymatic reactions.

  • 1M Tris-HCl
  • 100mM Ethylenediaminetetraacetic acid (EDTA)
  • Nucleic Acid Storage: Long-term storage of DNA and RNA at pH 8.0; EDTA chelates divalent cations to inhibit nucleases.

  • Nucleic Acid Resuspension: Rehydration and resuspension of purified plasmid DNA, genomic DNA, and RNA after precipitation or column elution.

  • PCR/qPCR Template Dilution: Stable dilution of DNA/RNA templates for PCR and qPCR while protecting against DNase/RNase activity.

  • Sequencing Sample Preparation: Resuspension and dilution of DNA for Sanger and NGS library inputs to maintain integrity and consistent pH.

  • Cloning Workflows: Safe storage of PCR amplicons and plasmids prior to ligation, transformation, or Gibson assembly (not as reaction buffer).

  • Oligonucleotide Handling: Reconstitution and storage of primers and probes to preserve stability and activity.

  • Plasmid Prep Elution Buffer: Elution and stock storage of miniprep/midiprep DNA from spin columns.

  • Colony PCR and Screening: Suspension of bacterial colony material (e.g., Escherichia coli) and stabilization of released DNA for PCR screening.

  • gDNA/cDNA Libraries: Storage and dilution of cDNA and gDNA libraries prior to quantification and downstream assays.

  • Sample Transport: Short-term transport of DNA/RNA between labs while minimizing nuclease-mediated degradation.

  • pH: 8.00 ± 0.10
  • DNase Free
  • RNase Free
Storage Temperature
Room Temperature
Shelf Life
1095 days
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