Environmental Monitoring (Cleanrooms): Recovers bacteria from air and surfaces in controlled areas; lecithin (0.07%) and Tween-80 (0.50%) help neutralize disinfectant residues to prevent false negatives.

Surface Hygiene Testing (Post-Disinfection): Used with swabs or contact methods to enumerate survivors after cleaning; formulation supports recovery in the presence of quats and phenolics.

Air Sampling by Impaction: Serves as a collection medium for viable airborne counts in pharmaceutical, biotech, and hospital environments.

Cleaning and Disinfection Validation: Verifies sanitizer efficacy and residue neutralization in qualification studies and routine verification.

Bioburden and Microbial Limits Testing: Cultures environmental and in-process samples where disinfectant carryover may inhibit growth on standard TSA.

Routine Environmental Surveillance (Food, Beverage, Cosmetics): Monitors facility hygiene and tracks trends in production areas exposed to sanitizers.

Recovery of Sanitizer-Stressed Organisms: Facilitates growth of bacteria such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Enterobacter cloacae after exposure to cleaning agents.

General Aerobic Plate Count: Supports broad aerobic heterotroph recovery with 1.50% Tryptone, 0.50% Soytone, 0.50% NaCl, and 1.50% Agar for robust growth.