Isolation and Cultivation of Clinical Bacteria: General-purpose growth of aerobic and facultative organisms from clinical specimens, including Streptococcus, Staphylococcus, and Enterococcus.

Hemolysis Differentiation: Visual assessment of alpha, beta, and gamma hemolysis for identification of species such as Streptococcus pyogenes (beta) and Streptococcus pneumoniae (alpha).

Colony Morphology Assessment: Evaluation of colony size, color, odor, and pigment production for organisms like Staphylococcus aureus and Pseudomonas aeruginosa.

Primary Plating in Clinical Microbiology: Initial recovery of potential pathogens from throat, wound, blood, and respiratory samples, including detection of Streptococcus pyogenes.

Satellite Test for Fastidious Organisms: Detection of Haemophilus influenzae via satellitism around Staphylococcus aureus streaks.

Veterinary and Food Microbiology Screening: Recovery and observation of hemolytic organisms relevant to animal health and food safety, such as Listeria monocytogenes.

Antimicrobial Screening (Where Applicable): Qualitative assessment of inhibition around agents on blood-supplemented surfaces for fastidious bacteria when standardized methods permit.

Quality Control of Hemolytic Phenotypes: Verification of expected hemolysis patterns using reference strains of Staphylococcus aureus, Streptococcus pyogenes, and related species.