Primary Isolation from Clinical Specimens: Enriched, nonselective medium for recovery of fastidious and nonfastidious bacteria from blood, throat, wound, and respiratory samples.

Hemolysis Differentiation: Assessment of alpha, beta, and gamma hemolysis patterns to help identify streptococci and related organisms (e.g., Streptococcus pyogenes, Streptococcus pneumoniae).

Colony Morphology Assessment: Observation of colony size, color, and hemolysis for organisms such as Staphylococcus aureus, Enterococcus faecalis, and Streptococcus agalactiae.

Throat Culture Screening: Detection of Group A Streptococcus pyogenes from pharyngeal specimens.

CAMP Test: Demonstration of enhanced hemolysis for identification of Streptococcus agalactiae in the presence of Staphylococcus aureus.

Subculture and Maintenance: Routine subculturing of clinical isolates that benefit from an enriched, blood-supplemented base.

Quality Control Verification: Performance testing with reference strains to confirm expected hemolysis reactions and growth characteristics.

Environmental and Product Monitoring: Recovery of aerobic bacteria, including hemolytic species, from surfaces, water, and process samples.

Differentiation of Respiratory Pathogens: Supportive growth and hemolysis observation for organisms encountered in sputum cultures (e.g., Streptococcus pneumoniae, Staphylococcus aureus).

General Microbial Enumeration (Enriched): Enhanced detection of stressed or fastidious bacteria compared to unsupplemented media.