Protein Storage and Dilution: Provides stable pH 6.8 buffering for proteins and antibodies using 200mM Sodium Phosphate, minimizing activity loss during handling.

Ion Exchange Chromatography: Used as equilibration and elution buffer at pH 6.8 for anion/cation exchange workflows, leveraging 200mM Sodium Phosphate for consistent ionic strength.

Enzyme Assays: Supplies a reliable near-neutral buffer (200mM Sodium Phosphate, pH 6.8) for enzymes that perform optimally in phosphate systems.

ELISA and Immunoassays: Employed as coating, blocking, or sample dilution buffer where a phosphate-based, sterile solution at pH 6.8 is preferred.

HPLC/SEC Mobile Phase: Utilized as a phosphate buffer for protein/peptide separations, offering reproducible retention and peak shape at pH 6.8.

PBS and Media Preparation: Serves as a concentrated stock to formulate PBS or phosphate-buffered microbial media (e.g., M9, LB-phosphate) for culturing Escherichia coli and other bacteria.

Western Blot Transfer (Phosphate-Based): Alternative transfer buffer to preserve phospho-epitopes, using 200mM Sodium Phosphate at pH 6.8 for consistent conductivity.