Cell and Tissue Lysis: Rapid extraction of total protein from cultured cells and tissues using a stringent ionic/non-ionic detergent mix.

Membrane and Nuclear Protein Solubilization: Efficient recovery of membrane-bound and nuclear proteins for downstream analyses.

Western Blot Sample Preparation: Generation of denatured protein lysates compatible with SDS-PAGE and immunoblotting.

Stringent Immunoprecipitation (IP): Lysis and high-stringency washing to reduce nonspecific binding in IP workflows.

Chromatin Immunoprecipitation (ChIP) Lysis/Washes: Harsh lysis and washes for ChIP protocols to minimize background.

Phosphoprotein Studies: Preparation of lysates for phosphorylation analysis when supplemented with protease/phosphatase inhibitors.

Bacterial Cell Lysis: Lysis of organisms such as Escherichia coli with additional enzymatic or mechanical disruption as needed.