Antimicrobial Susceptibility Testing (AST): Standard medium for broth microdilution/macrodilution and disk diffusion inoculum prep; cation adjustment (Ca2+/Mg2+) supports reproducible MICs, especially for aminoglycosides, tetracyclines, and polymyxins.

MIC/MBC Determination: Used to measure inhibitory and bactericidal endpoints for clinical isolates.

Cultivation of Nonfastidious Bacteria: Supports robust growth of Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis.

Quality Control Testing: Employed with ATCC reference strains for media performance and antibiotic potency QC.

Antibiotic Screening and Lot Release: Used to evaluate activity of new compounds and verify batch-to-batch consistency.

Growth Kinetics and Inoculum Preparation: Generates standardized cell densities for downstream assays and time-kill studies.

Supplemented AST for Fastidious Organisms: Serves as a base for supplemented media (e.g., HTM) for testing Haemophilus influenzae and certain streptococci.