Methanol-Induced Expression in Pichia pastoris (Komagataella phaffii): Enables AOX1-driven production of recombinant proteins during induction in shake flasks and bioreactors.

Secreted Protein Production: Supports secretion workflows (e.g., α-factor signal) for extracellular protein yield and simplified downstream purification.

Clone Screening and Titer Ranking: Used in deep-well plates and flasks to assess expression levels of multiple transformants under standardized methanol induction.

Enzyme Manufacturing: Facilitates high-level expression of industrial and research enzymes under methanol utilization conditions.

Process Development and Optimization: Provides a buffered environment for refining methanol feed profiles, induction timing, and temperature to maximize yield.

AOX Pathway and Metabolism Studies: Suitable for assays of alcohol oxidase activity and investigations of methanol metabolism and stress responses.

Glycoprotein and Membrane Protein Expression: Supports production of complex eukaryotic proteins requiring secretory pathways or membrane insertion.

Agar Plate and Liquid Induction: Used to prepare induction plates and liquid cultures for rapid expression checks and colony-level screening.