Antibiotic Selection of Transformants: Recovery and selection of ampicillin-resistant Escherichia coli after chemical or electroporation transformation.

Plasmid Maintenance/Propagation: Stable maintenance of AmpR plasmids in Escherichia coli on LB agar with 100 µg/mL ampicillin.

Reduced Basal Expression During Cloning: 1% glucose minimizes leaky expression from lac/T7-lac promoters, aiding cloning of toxic inserts in Escherichia coli.

Colony Isolation and CFU Enumeration: Isolation of single colonies and counting of ampicillin-resistant colony-forming units.

Colony PCR/Screening: Picking colonies for PCR, restriction analysis, or sequencing to verify constructs.

Library Plating: High-density plating of combinatorial, mutagenesis, or genomic libraries on 150 mm plates.

Replica Plating: Transferring selected colonies to secondary selective conditions or induction plates.

Strain Archiving and Picking: Picking verified clones for liquid culture inoculation and glycerol stock preparation.

Conjugation/Mating Selections: Selecting AmpR plasmid recipients or transconjugants in Escherichia coli.

Contamination Control: Suppressing Amp-sensitive contaminants while maintaining desired AmpR Escherichia coli clones.