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H8174

10X HBS-P Buffer, pH 7.4 at 1X. 1000mL, Sterile

Availability

Ships in 1 - 2 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$88.00

Fill Volume

1000 mL

H8174

10X HBS-P Buffer, pH 7.4 at 1X. 1000mL, Sterile

Quantity

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Description
Formulation
Common Applications
Storage

10X HBS-P Buffer is a concentrated HEPES-buffered saline with a nonionic surfactant, supplied at 10X strength for dilution to a 1X working solution at pH 7.4.

Prepare 1X by diluting 1 part 10X buffer with 9 parts suitable water.

  • 1.5M Sodium Chloride
  • 0.1M HEPES
  • 0.5% Tween-20
  • Surface Plasmon Resonance (SPR): Standard running buffer for biosensor assays, maintaining ionic strength and reducing nonspecific binding.

  • ELISA: Wash and sample/antibody dilution buffer to improve signal-to-noise in plate-based immunoassays.

  • Western Blot: Wash buffer and antibody diluent to minimize background during immunodetection.

  • Immunoprecipitation/Affinity Purification: Wash buffer to preserve specific interactions while limiting nonspecific binding.

  • Flow Cytometry: Staining and dilution buffer for cells and antibodies at physiological pH.

  • Protein–Protein/Ligand Binding Assays: Assay buffer for kinetic and equilibrium interaction studies.

  • Cell-Based Assays: Physiological pH buffering for live-cell applications and compound screening.

  • Microarrays/Bead-Based Assays: Wash and hybridization buffer to reduce nonspecific interactions.

  • Sample Preparation: Protein dilution and short-term storage buffer to maintain stability.

  • Plate Washing: General wash buffer for high-binding and low-binding assay plates.

Storage Temperature
Room Temperature
Shelf Life
1095 days
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