Microbial Culture Supplementation: Added as a concentrated carbon source to media for high-density growth of Escherichia coli, Bacillus subtilis, and other bacteria.

Fed-Batch Fermentation: Used as a sterile feed to control carbon supply and prevent overflow metabolism in bacterial and yeast bioprocesses.

Defined/Minimal Media Preparation: Provides precise glucose concentrations in M9 and other minimal media formulations.

Agar Plate Preparation: Supplementation of plates (e.g., LB-glucose, minimal agar) to support growth and selection of Escherichia coli and other microbes.

Mammalian Cell Culture Supplementation: Adjustment of glucose levels in basal media (e.g., preparing high-glucose DMEM) to meet metabolic demands.

Auto-Induction and Expression Media: Component of auto-induction and regulated feeding strategies for recombinant protein expression in Escherichia coli.

Enzyme and Metabolic Assays: Substrate for glycolytic and glucose-utilizing enzyme assays (e.g., hexokinase, glucose oxidase).

Osmotic Stress Studies: Controlled elevation of osmolarity to study cellular responses in bacteria such as Escherichia coli and Pseudomonas aeruginosa.

Yeast Culture and Fermentation: Primary carbon source for growth and ethanol production in Saccharomyces cerevisiae.

Bioreactor Glucose Replenishment: On-demand dosing to maintain target glucose setpoints during continuous and perfusion runs.

Diagnostic and QC Procedures: Preparation of standards and controls for glucose meters and biochemical analyzers.

Antibiotic Production and Secondary Metabolism: Carbon source tuning in fermentation of producers such as Streptomyces spp.