Culture Media Supplementation: Added as a primary carbon source for minimal media and agar/broth formulations.

Fed-Batch Fermentation Feeds: Concentrated carbon feed to control growth rates and productivity in bioreactors.

SOC/SOB Medium Enhancement: Supplemented in SOC to improve post-transformation recovery of Escherichia coli.

Mammalian Cell Culture: Adjusts glucose levels in basal media and supports starvation/re-feeding experiments.

Yeast Cultivation: Carbon source for high-density growth and metabolic studies.

Analytical Standards: Preparation of calibration curves for glucose oxidase/hexokinase assays and instrument verification.

Osmotic Modulation: Used to adjust medium osmolarity for osmotic stress and tolerance studies.

Protein/Enzyme Stabilization: Sugar excipient to stabilize biomolecules during storage or lyophilization.

Post-Sterilization Supplementation: Aseptically added to autoclaved media to avoid glucose degradation.

Metabolic Flux Experiments: Precise carbon input for glycolysis and catabolite regulation studies.