Cryopreservation of Bacterial Stocks: 50% (v/v) Glycerol is mixed with cultures to achieve 15–25% final glycerol for long-term freezer stocks of E. coli and other bacteria/yeast.

Electrophoresis Sample Loading: Added to DNA/RNA/protein loading dyes to increase density and viscosity for reliable well loading in gels.

Enzyme and Protein Stabilization: Included in storage buffers to reduce aggregation, prevent freezing damage, and maintain activity during freeze–thaw cycles.

PCR Additive: Used in PCR to improve amplification of GC-rich templates and reduce secondary structures; added to reactions at low percent final glycerol.

Cell Cryoprotection: Serves as a cryoprotectant for mammalian and insect cells during controlled-rate freezing and storage.

Reagent and Column Storage: Included in antibody and chromatography resin buffers to prevent freezing and enhance stability during refrigerated storage.

Media Supplementation (Carbon Source): Sterile 50% (v/v) Glycerol is aseptically added to culture media for organisms such as E. coli, Pseudomonas, and Bacillus.

Glycerol Gradients: Used to prepare density gradients for macromolecule separation by ultracentrifugation.