First-Strand cDNA Synthesis: Converts RNA templates into cDNA using reverse transcriptase for downstream analyses.

RT-PCR: Generates cDNA for endpoint RT-PCR amplification of RNA targets.

qRT-PCR: Enables quantitative gene expression analysis by producing high-quality cDNA for real-time PCR.

RNA-Seq Library Preparation: Supports first-strand cDNA synthesis during transcriptome library construction.

cDNA Library Construction and Cloning: Produces stable first-strand cDNA for cloning and sequencing workflows.

5' and 3' RACE: Facilitates rapid amplification of cDNA ends to map transcript boundaries.

Primer Extension Assays: Provides conditions for reverse transcriptase-based mapping of transcription start sites.

miRNA/lncRNA Analysis: Supports reverse transcription of small and long non-coding RNAs for downstream detection.

One-Step RT-PCR Workflows: Functions as the RT component buffer in combined reverse transcription and PCR protocols.

Viral RNA Detection: Used to synthesize cDNA from RNA viruses for diagnostic amplification assays.