Protein Disulfide Reduction: Reduces cystine bonds in proteins and peptides for sample preparation.

SDS-PAGE and Western Blotting: Ensures complete protein denaturation and maintains reduced cysteines during electrophoresis.

Protein Purification and Storage: Maintains enzymes and proteins in a reduced, active state to prevent oxidation.

Reverse Transcription and cDNA Synthesis: Stabilizes reverse transcriptases and RNase inhibitors by providing reducing conditions.

PCR and qPCR: Supports enzyme stability in reactions that benefit from a reducing environment.

RNA Workflows: Protects RNA by reducing thiol-dependent RNases during extraction and handling.

Proteomics (LC-MS/MS): Reduction prior to alkylation and tryptic digestion to prevent artifactual disulfide formation.

Enzyme Assays: Provides reducing conditions for thiol-dependent enzymes to ensure accurate kinetics.

Deglycosylation Protocols: Facilitates denaturation and reduction prior to PNGase F digestion.

Thiol Labeling and Conjugation: Prepares free thiols for maleimide or iodoacetamide-based modification.