Recombinant Protein Expression (Pichia pastoris/Komagataella phaffii): Methanol-induced AOX1-driven expression in shake flasks, deep-well plates, and bioreactors.

Clone Screening: Rapid evaluation of methanol-inducible clones for expression levels and stability.

Induction Phase Medium: Switch from glycerol-based growth (e.g., BMGY) to methanol-based induction for target protein production.

Secreted Protein Production: Supports secretion signal screening and high-yield extracellular protein recovery.

Intracellular Protein Production: Enables high-cell-density induction for intracellular target accumulation and downstream lysis.

Strain and Promoter Characterization: Assessment of Mut+ vs. MutS phenotypes and AOX1/AOX2 promoter performance under methanol induction.

Process Optimization: Tuning temperature, methanol feed, and pH during induction to maximize titer and quality.

Enzyme and Antigen Production: Generation of enzymes, vaccine antigens, and assay reagents for R&D and preclinical workflows.

Glycoprotein Expression: Production of yeast-glycosylated proteins for functional studies and comparability assessments.

Method Development and Scale-Up: Bridging small-scale expression to pilot/production fermentations with controlled methanol induction.