Yeast Cultivation: Buffered rich medium for robust growth and propagation of Saccharomyces cerevisiae and other yeasts.

Yeast Transformation Outgrowth: Supports recovery and expansion after transformation or genome editing, aided by balanced nutrients (2.0% Tryptone, 1.0% Yeast Extract) and phosphate buffering.

Recombinant Protein Expression (Yeast): Enables high-density, pH-stable cultures for expression and secretion studies.

Fermentation and Metabolite Production: Phosphate-buffered medium (KH2PO4/K2HPO4) sustains consistent pH during small-scale fermentations.

Physiology and Growth Kinetics: Suitable for growth curves, stress assays, and nutrient response studies using Yeast Nitrogen Base and biotin supplementation.

Bacterial Cultivation: Supports routine growth of Escherichia coli and related lab strains in a buffered complex environment.

Starter Culture Preparation: Used to generate inocula for bioreactors, plates (with agar), or downstream assays when supplemented with a carbon source.

Glycerol Stock Preparation: Nutrient-rich, buffered medium for preparing stable microbial freezer stocks.