Antibody Conjugation and Labeling: Ideal amine-free buffer at pH 8.3 for NHS-ester dye/biotin coupling to antibodies; dilute 10X (100mM Boric Acid, 1.5M Sodium Chloride) to 1X for optimal ionic strength.

Immunoassay Diluent and Wash Buffer: Used as a 1X diluent/wash in ELISA and other immunoassays to maintain stable pH 8.3 without Tris interference.

Protein Immobilization and Bead Coupling: Supports covalent attachment of proteins to activated resins or surfaces at mildly alkaline pH.

Glycoprotein and Carbohydrate-Related Workflows: Borate interacts with cis-diols, aiding in workflows involving glycoproteins and carbohydrates.

Enzyme Reactions Sensitive to Primary Amines: Provides a consistent pH 8.3 environment where Tris would inhibit amine-reactive chemistries.

Microarray and Slide Printing: Used for spotting/printing antibodies and proteins onto slides while maintaining structure and activity at 1X ionic strength.

General Sample Preparation and Storage: Serves as a 1X buffer for protein/nucleic acid handling where stable pH 8.3 and physiological ionic strength are required.