Proteomic Digestions: Buffer for in-solution and in-gel trypsin digestion of proteins using 50 mM Ammonium Bicarbonate, providing near-neutral pH and volatility for MS compatibility.

LC-MS/MS Sample Prep: Volatile buffer for peptide preparation and cleanup, minimizing non-volatile salts prior to mass spectrometry.

Solid-Phase Extraction (SPE): Used during peptide desalting and elution steps where a volatile buffer is required for downstream MS.

Buffer Exchange/Cleanup: Exchange into 50 mM Ammonium Bicarbonate before lyophilization or MS to facilitate complete removal by drying.

Reduction/Alkylation Workflows: Matrix for DTT reduction and IAA alkylation prior to enzymatic digestion in proteomics.

Peptide Mapping: Standard buffer for bottom-up proteomic workflows to generate peptide maps compatible with LC-MS analysis.

Enzymatic Assays: Provides controlled, near-neutral pH for protein-modifying enzymes where a volatile buffer is advantageous.

HPLC/LC Mobile Phase Additive: Employed at low concentrations as a volatile buffering component for peptide separations in LC-MS.

Sample Reconstitution: Reconstituting dried peptide/protein digests in 50 mM Ammonium Bicarbonate before further processing.

Lyophilization-Friendly Formulations: Preparing samples in a fully volatile buffer to enable residue-free drying and improved MS sensitivity.