Routine Cultivation of Escherichia coli: General growth and maintenance of lab strains for molecular biology workflows.

Plasmid Cloning and Amplification: Selection and propagation of plasmids in recombinant Escherichia coli with antibiotics.

Protein Expression: Growth medium for recombinant protein production in Escherichia coli (shake flasks and bench bioreactors).

Starter Culture Preparation: Inoculum development for downstream fermentation or scale-up.

Transformation Recovery: Post-chemical or electroporation outgrowth of competent Escherichia coli cells.

Growth Curves and QC: OD600 measurements, strain fitness comparisons, and media performance checks.

Glycerol Stock Preparation: Long-term storage of bacterial libraries and engineered strains.

Phage Workflows: Amplification of M13 and other coliphages using host Escherichia coli.

Non-Fastidious Bacteria Culture: General propagation of organisms such as Bacillus subtilis under standard conditions.

Animal-Component-Free Processes: Microbial applications requiring animal-free components for regulatory or compliance needs.