Routine Cultivation of Escherichia coli: General-purpose growth of E. coli and other non-fastidious bacteria in liquid culture.

Plasmid Propagation: Growing cultures for plasmid minipreps, midipreps, and maxipreps.

Overnight/Starter Cultures: Preparing inocula for cloning, subcloning, and downstream fermentations.

Protein Expression Pre-cultures: Building seed cultures prior to induction in expression workflows.

Antibiotic Selection: Maintaining and selecting plasmid-bearing strains when supplemented with appropriate antibiotics.

Transformation Recovery: Short post-transformation outgrowth after chemical or electroporation methods.

Strain Maintenance: Routine passaging and storage culture preparation for lab strains.

Glycerol Stock Preparation: Growing cells to mid/late log for long-term cryostorage.

Growth Curves and OD600 Monitoring: Assessing bacterial growth kinetics and media performance.

Phage-Resistant Host Cultivation: Culturing hosts prior to phage-related assays when appropriate.

Competent Cell Expansion: Pre-growth of cells intended for chemical or electrocompetent preparation.

Broad Bacterial Use: Culturing non-fastidious species such as Bacillus subtilis and Pseudomonas fluorescens for routine lab applications.