Polymerase Chain Reaction (PCR) and qPCR: Maintains optimal alkaline pH in reaction buffers to support polymerase activity and fidelity.

Gel Electrophoresis: Component of Tris-based running and tank buffers (e.g., Tris-glycine, TAE/TBE) for consistent pH during DNA/RNA and protein separation.

Protein Purification: Used in chromatography buffers (e.g., anion exchange) to stabilize pH during binding, washing, and elution.

Enzyme Assays: Provides controlled pH for alkaline-range enzymatic reactions and kinetic studies.

Western Blotting: Included in transfer and running buffers to maintain pH during protein transfer and detection.

Sample and Cell Lysis Buffers: Forms the buffering backbone for lysis and extraction solutions to preserve biomolecule integrity.

Nucleic Acid Workflows: Buffering component for preparation and dilution of DNA/RNA solutions and restriction/ligation setups as required.

Immunoassays (e.g., ELISA): Used in coating, blocking, and wash buffers to maintain assay performance and reproducibility.

pH Adjustment and Buffer Preparation: Convenient sterile 1M stock for rapidly setting and fine-tuning Tris-based buffers near pH 9.0.

Endotoxin-Sensitive Applications: Endotoxin-tested, sterile buffer stock suited for workflows feeding into cell-based and bioproduction assays.