Polymerase Chain Reaction (PCR/qPCR): Used to prepare and adjust PCR buffer systems to pH 8.0 for optimal polymerase activity.

Gel Electrophoresis: Base for making TAE/TBE running and loading buffers, maintaining stable pH during DNA/RNA separation.

Nucleic Acid Extraction and TE Buffer: Provides the Tris component for DNA/RNA lysis, wash buffers, and TE (Tris-EDTA) for nucleic acid resuspension and storage.

Protein Purification: Used to formulate equilibration, wash, and elution buffers in chromatography and affinity purification at controlled pH.

Enzyme Assays: Supplies a stable pH 8.0 environment for restriction digests, ligations, kinase/phosphatase reactions, and other enzymatic assays.

Western Blotting: Component of transfer buffers and Tris-based saline buffers (e.g., TBS) for membrane blocking and antibody incubations.

Cell Lysis and Immunoprecipitation: Forms the buffering base for RIPA and other lysis/IP buffers to preserve protein integrity and activity.

Endotoxin-Sensitive Workflows: Sterile, endotoxin-tested stock for preparing buffers used with primary cells and plasmids purified from Escherichia coli to minimize LPS interference.

General Buffer Preparation: Convenient 1 M stock for rapid pH adjustment and dilution to common working concentrations (e.g., 10–100 mM Tris-HCl, pH 8.0).