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21325

1M Tris-HCl, pH 7.5 Solution, 1000mL, Sterile.

Availability

Ships in 6 - 14 Days

Container Size

1000mL

Item Count

1 Bottle(s)

Price per Item

$85.00

Fill Volume

1000 mL

21325

1M Tris-HCl, pH 7.5 Solution, 1000mL, Sterile.

Quantity

Minimum is set to 2 for this product*

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Description
Formulation
Common Applications
Quality Specifications
Storage

1 M Tris-HCl, pH 7.5 Solution is a ready-to-use buffering reagent for preparing and maintaining pH in biological and biochemical workflows. At pH 7.5, it provides stable buffering capacity suitable for nucleic acid handling, protein work, and enzymatic reactions.

This solution can be used to prepare common laboratory buffers (e.g., TE, TAE, and TBE), to adjust and maintain pH in lysis and wash solutions, and to formulate reaction buffers where Tris at neutral to slightly basic pH is required. GMP manufactured.

  • This is a GMP-grade product that is manufactured in compliance with our Quality Management System in ISO 13485 certified facilities.
  • 1M Tris
  • Polymerase Chain Reaction (PCR/qPCR): Used to prepare reaction buffers and maintain stable pH for polymerase activity.

  • Gel Electrophoresis: Component in preparing Tris-based buffers (e.g., TAE/TBE, Tris-glycine) for nucleic acid and protein separation.

  • Protein Purification: Utilized in ion-exchange and affinity chromatography buffers to maintain pH during binding and elution.

  • Enzyme Assays: Provides consistent pH for enzymatic reactions and kinetic measurements.

  • DNA/RNA Workflows: Used in TE and resuspension buffers for nucleic acid extraction, storage, and handling.

  • Cloning Workflows: Supports restriction digests, ligations, and competent cell transformations.

  • Western Blotting: Used in Tris-buffered saline (TBS) and Tris-glycine transfer buffers.

  • Bacterial Lysis and Plasmid Prep: Included in lysis and wash buffers for plasmid purification from E. coli.

  • GMP Bioprocessing: Suitable for aseptic upstream/downstream buffer prep and formulation in regulated workflows.

  • This is a GMP-grade product that is manufactured in compliance with our Quality Management System in ISO 13485 certified facilities.
  • pH (USP <791>)
  • Conductivity (USP <644>)
  • Endotoxin (USP <85>)
  • Osmolality (USP <785>)
  • Density (USP <841>)
  • Sterility (USP <71>)
Storage Temperature
Room Temperature
Shelf Life
1095 days
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