Sample Dilution and Reconstitution: Sterile diluent for reconstituting lyophilized reagents, controls, and standards where reduced ionic strength is preferred.

Cell and Tissue Rinsing: Gentle hypotonic rinse for cells and tissues to remove residual media, serum, or inhibitors without introducing high salt.

Hypotonic Cell Swelling: Used to induce controlled osmotic swelling of mammalian cells and nuclei for cytogenetics and permeability studies.

Erythrocyte Lysis/Leukocyte Enrichment: Facilitates hypotonic lysis of red blood cells to enrich white blood cells prior to downstream assays.

Microbial Pellet Washing: Brief, low-ionic-strength wash for bacterial pellets (e.g., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa) prior to extraction or analysis.

Instrument and Syringe Flushing: Sterile rinse for laboratory tubing, cannulas, and syringes to minimize contamination between samples.

Specimen Transport and Handling: Temporary sterile carrier for swabs or small tissue pieces when minimal salt is desired before processing.

Protein and Enzyme Workflows: Low-ionic-strength medium for diluting proteins or enzymes to reduce ionic interference in sensitive assays.