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AEX Buffer Screening Kit

Discover our solutions

Our proprietary AAV●Tek™ Solutions are designed to remove obstacles in your gene therapy workflow and help you seamlessly scale to commercial manufacturing.

The inaugural solution is a first-of-its-kind AEX Buffer Screening Kit that can help you find the ideal buffer to optimize the separation of empty and full capsids while maintaining high yields. It can save months in your AAV2 process development at the polishing step.

AEX buffer screening kit with box
workflow workflow
AEX Buffer Screening Kit: AAV2

Our first AAV-Tek solution is a proprietary off-the-shelf AEX Buffer Screening Kit for AAV2.

Through extensive screening, we created a first-of-its-kind kit of equilibration and elution buffers. It can help you identify the ideal buffer formulation to optimize the separation of empty and full capsids, saving you months in your process development at the polishing step.


This state-of-the-art kit contains 7 paired sets of 1 L bottles (14 bottles total). These discrete formulations have been proven to be effective across multiple upstream and purification platforms.  





AAV polishing, 


With over 25 years of experience manufacturing custom buffers and a dedicated Research and Development team with expertise in the AAV bioproduction process, we can help simplify your AAV workflow.

Save months of development time


When you are building a new process, it requires significant investment to find the right reagents to use across your workflow. Our kit accelerates this process, saving you weeks of trial and error so you can make better use of your time and resources.

Optimize capsid separation

Effectively separate empty and full capsids. Our rigorous testing and batch-to-batch consistency help you get results you can trust. The kit has been verified for use on multiple upstream workflows, transgenes, and downstream purification platforms.

Seamless scale-up from RUO to GMP

Our scientists and engineers have the expertise to guide you through the end-to-end buffer screening process. Once you find the ideal fit for your system, we can help make further customizations and set you up with a reliable supply of your unique formulation.

See the data

To identify serotype-specific buffer formulations, we utilized a Design of Experiments (DOE) approach to define the most impactful attributes for the polishing step of your AAV2 purification process. These optimized buffers are designed to effectively remove empty capsids while maintaining maximum recovery and infectivity.

The process parameters evaluated for AAV2 full capsid enrichment include a full range of pH, elution salts, excipients, surfactants, and stabilizers/osmolytes. The DOE was executed to elucidate the extent of each contributing factor.

Implementation of DOE was used to determine optimal buffer conditions for this particular serotype. In vitro data has shown that effective separation of empty and full capsids can be performed using this buffer kit.


Different DNA sequences that are packaged within the viral capsid may impact the pI of the overall construct. Therefore, the optimized buffer formulations were validated against multiple sequences, as shown in these figures.


Initial work implementing the DOE was performed using monolith technology due to the high flow rates associated with these columns. After identification of the optimal recipe, however, the formulation was validated across multiple other platforms, including two commonly used AEX resins shown in the figure below.


To ensure the kit is agnostic of the upstream process used (for instance, cell line, media choice, transfection and harvest conditions) and does not affect the ability to successfully utilize the buffer set, multiple processes were implemented and tested using the optimal buffer formulations.


Variations to the downstream processes, such as TFF, affinity, and CEX, may affect the final AEX polishing step. To probe this concern, multiple downstream processes were implemented to verify the applicability of the optimized buffer formulations.


The figure below demonstrates the utility of the kit for various chromatographic modalities. The buffer screening is shown using a linear gradient (left) and then finalized using an isocratic hold (right) to facilitate scale-up requirements.



The protocol

These instructions will guide you through the process of using the AEX Buffer Screening Kit so you can get optimal results. Have a question? We're here to help. Drop us a line at or call 1.800.209.4488.

What's next

From screening to scale-up

Our AEX Buffer Screening Kit sets you up to scale for commercial production. It addresses a key challenge for gene therapy manufacturing by identifying the optimal buffer for the removal of empty capsids from the therapeutic product while still maintaining high yields.


After using the kit to select the buffer that best fits your specific application, consult with our team to get set up to scale. We can help make further customizations as needed and assist with GMP-compliant manufacturing.


Product guide

Here's a quick reference sheet with all the key information
about our AEX Buffer Screening Kit for AAV2.

AAV●Tek Solutions

More serotypes, coming soon

We are in the process of developing screening solutions for AAV6, AAV8, and AAV9. If you are interested in trying these products, talk to our team today about joining our Early Access Program.

Join our Early Access Program


We are actively looking for partners to participate in our Early Access Program. You'll gain exclusive access to products currently in development and have the opportunity to provide feedback that can shape their design. Get in touch with us at to learn more and join the program.

The people behind our novel products

Meet the AAV team

Our AAV●Tek Solutions are designed by our dedicated Research and Development team as part of our initiative to invest in the discovery of novel products designed to give you a competitive advantage in your work.


With custom manufacturing capabilities that fit seamlessly into your cell and gene therapy workflow and our in-house scientific expertise, we can be a true partner to you, helping you overcome bioprocessing challenges and accelerate your breakthroughs.

Group shot of the research and development team

Find out what’s possible


Talk to our consultants today to discover custom solutions that can help you achieve your goals.


Get in touch:


Monday to Friday

8:00AM–5:00PM PT

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