Gene therapy holds promise for improving the lives of people with genetic diseases. To enable effective adoption of these novel therapies, there is a need to develop scalable, cost-effective methods for viral vector production. However, current processes for manufacturing gene therapy products are not standardized.

Teknova partners with you to develop and optimize your gene therapy production workflow from upstream plasmid production and cell culture through downstream purification and final production (Figure 1).

AAV workflow for gene therapy

Figure 1. Schematic overview of viral vector–based gene therapy manufacturing.


Media, Buffers, and Solutions for Upstream Processes


Select from a wide variety of liquid and solid media for plasmid production and cell expansion, create your own custom media, or modify existing media to suit your needs.

Molecular Biology Buffers

Let us manufacture your custom formulations or you can select from common buffers for the various steps in your plasmid production and purification.

  • PCR buffers
  • T7 buffer
  • TE buffers
  • Tris buffers
  • Water for molecular biology

Plasmid Purification Solutions

Explore our website for common solutions, including acids and bases, sugar solutions, detergents, antibiotics, glycerol, and more. Other solutions include:

  • Dithiothreitol (DTT)
  • Lysis buffers
  • Ammonium sulfate with TE
  • NaCl with TE

Viral Vector Production Buffers

Start with our extensive range of common buffers or request custom buffers unique to your vector serotype, including:

  • Buffers with Pluronic® polymer*
  • PBS
  • Sodium bicarbonate

Viral Vector Production Solutions

Select from a wide variety of solutions to support your viral vector production. In addition to common solutions such as NaCl and HEPES, we offer:

  • Glucose
  • Polyethylene glycol (PEG) 8000
  • Phosphate buffered saline (PBS)

Buffers and Solutions for Downstream Processes

Viral Vector Purification Buffers

Explore our extensive selection of buffers, including MES buffers; sodium acetate, sodium citrate, and sodium phosphate buffers; and Tris buffers. Also, partner with us to formulate and optimize custom buffers for:

  • Ion exchange chromatography (IEC)
  • Affinity chromatography
  • Hydrophobic interaction chromatography (HIC)
  • Lysis
  • Column cleaning (CIP, cleaning-in-place)

Viral Vector Purification Solutions

Choose from common concentrations of common solutions or have us create custom concentrations for your needs:

  • Acetic acid
  • Sodium citrate buffers
  • Concentrated sucrose
  • Phosphate-buffered sucrose
  • Iodixanol
  • WFI quality water

Contact Us

To enquire about options for fill volume, containers, and testing, or to request custom formulations, contact us at

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* Pluronic is a registered trademark of BASF Corp.