Selected Articles in Protein Expression

Selected Articles in Protein Expression

Protein expression in multiple systems

  1. Berrow NS, Alderton D, Sainsbury S, Nettleship J et al. A versatile ligation-independent cloning method suitable for high-throughput expression screening applications. Nucleic Acids Res.35(6): 45. (2007).  PMID: 17317681
  2. Brondyk, W.H. Selecting an Appropriate Method for Expressing a Recombinant Protein. Methods Enzymol. 463:131-47. (2009).  PMID: 19892171
  3. Scholz J, Besir H, Strasser C, Suppmann S. A new method to customize protein expression vectors for fast, efficient and background free parallel cloning. BMC Biotechnol. Feb 14;13:12 (2013). PMID: 23410102

Expression in E. coli

  1. Cabrita, L.D., Dai, W. Bottomley, S.P. A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotech. March 1:6:12. (2006).  PMID: 16509985
  2. de Marco A, Deuerling E, Mogk A,Tomoyasu T, Bukau B. Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli. BMC Biotechnol. Jun 12;7:32: 1-9. (2007) PMID: 17565681
  3. Francis DM, Page R. Strategies to optimize protein expression in E. coli. Curr Protoc Protein Sci. Aug; Chapter 5: Unit 5.24.1-29 (2010). PMID: 20814932
  4. Nannenga BL, Baneyx F. Folding engineering strategies for efficient membrane protein production in E. coli. Methods Mol Biol. 899:187-202 (2012). PMID: 22735954
  5. Nozach H, Fruchart-Gaillard C, Fenaille F, Beau F, Ramos OH, Douzi B, et al. High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli. Microb Cell Fact. Apr 22;12:37 (2013). PMID: 23607455
  6. Unger, T., Jacobovitch, Y., Dantes, A., Bernheim, R., Peleg, Y. Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression. J Struct Biol. Oct;172(1) 34-44. (2010). PMID: 20600952

Expression in Yeast

  1. Bollok, M., Resina, D., Valero, F. and Ferrer, P. Recent patents on the Pichia pastoris expression system: expanding the toolbox for recombinant protein production. Recent Patents on Biotechnology 3, 192-201 (2009). PMID: 19747151
  2. Cregg, J.M. Tolstorukov I, Kusari A, Sunga J, Madden K, Chappell T. Expression in the yeast Pichia pastoris. Methods in Enzymology 463, 169-189 (2009). PMID: 19892173
  3. Gonçalves AM, Pedro AQ, Maia C, Sousa F, Queiroz JA, Passarinha LA. Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins. J Microbiol Biotechnol. May;23(5):587-601 (2013). PMID: 123648847
  4. Hamilton, S.R. and Gerngross, T.U. Glyosylation engineering in yeast: the advent of fully humanized yeast. Current Opin Biotech. Oct 18(5): 387-392 (2007). PMID: 17951046
  5. Rabert C, Weinacker D, Pessoa A Jr, Farías JG. Recombinant proteins for industrial uses: utilization of Pichia pastoris expression system. Braz J Microbiol. Oct 30;44(2):351-356 (2013). PMID: 24294221
  6. Weidner M, Taupp M, Hallam SJ.Expression of recombinant proteins in the methylotrophic yeast Pichia pastoris. J Vis Exp Feb 25;(36) (2010). PMID: 20186119

Insect cell and Baculovirus expression

  1. Cremer H, Bechtold I, Mahnke M, Assenberg R. Efficient processes for protein expression using recombinant baculovirus particles. Methods Mol Biol.1104:395-417. (2014). PMID: 24297428
  2. Jarvis, D.L. Baculovirus-insect cell expression systems. Methods Enzymol. 463, 191-222 (2009). PMID: 19892174
  3. Kost, T.A., Condreay, J.P. and Jarvis, D.L. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nature Biotechnol. 23, 567-575 (2005). PMID: 15877075
  4. Philipps B, Rotmann D, Wicki M, Mayr LM, Forstner M. Time reduction and process optimization of the baculovirus expression system for more efficient
    recombinant protein production in insect cells. Protein Expr Purif.Jul;42(1):211-8. (2005) PMID: 15939308
  5. Possee RD, Hitchman RB, Richards KS, Mann SG, et al. Generation of baculovirus vectors for the high-throughput production of proteins in insect cells. Biotechnol Bioeng. Dec 15;101(6):1115-22 (2008). PMID: 18781697
  6. Trowitzsch, S., Bieniossek, C., Nie,Y., Garzoni, F. Berger, I. New baculovirus expression tools for recombinant protein complex production. J Struct Biol. Oct. 172(1), 45-54 (2010). PMID: 20178849
  7. Vijayachandran LS, Viola C, Garzoni Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells. J Struct Biol. Aug;175(2):198-208 (2011). PMID: 21419851

Mammalian Expression

  1. Baldi, L., Hacker, D.L., Adam, M., Wurm, F.M. Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives. Biotechnology Letters 29, 677-684 (2007). PMID: 17235486
  2. Geisse S, Fux C. Recombinant protein production by transient gene transfer into Mammalian cells. Methods Enzymol. 463, 223-238 (2009).
    PMID: 19892175
  3. Kahlig KM, Saridey SK, Kaja A, Daniels MA, George AL, Wilson MH. Multiplexed transposon-mediated stable gene transfer in human cells. Proc Natl Acad Sci. Jan 26;107(4):1343-8 (2010). PMID: 20080581
  4. Matasci M., Baldi L, Hacker DL, Wurm FM. The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability. Biotechnol Bioeng. Sep;108(9):2141-50 (2011). PMID: 21495018
  5. Matasci M, Bachmann V, Baldi L, Hacker DL, De Jesus M, Wurm FM. Rapid recombinant protein production from pools of transposon-generated CHO cells. BMC Proc. Nov 22;5 Suppl 8:P34(2011). PMID: 22373498
  6. Oberbek A, Matasci M, Hacker DL, Wurm FM. Generation of stable, high-producing CHO cell lines by lentiviral vector-mediated gene transfer in serum-free suspension culture. Biotechnol Bioeng. Mar;108(3):600-10 (2011). PMID: 20967750
  7. Walsh, G. Biopharmaceutical benchmarks. Nature Biotechnology 28, 917-924 (2010).PMID: 20829826