Complete Minimal (CM) Dropout Media: Formulation
Per Liter:
- 1.3 g dropout powder
- 1.7 g YNB-AA/AS*
- 5 g (NH4)2SO4
- 20 g dextrose
| Final concentration of nutrient in prepared media (µg/mL) | |
|---|---|
| Adenine (hemisulfate salt) | 40 |
| L-aspartic acid | 100 |
| L-arginine (HCl) | 20 |
| L-glutamic acid (monosodium salt) | 100 |
| L-histidine | 20 |
| L-leucine | 60 |
| L-Lysine (mono-HCl) | 30 |
| L-methionine | 20 |
| L-phenylalanine | 50 |
| L-serine | 375 |
| L-threonine | 200 |
| L-tryptophan | 40 |
| L-tyrosine | 30 |
| L-valine | 150 |
| Uracil | 20 |
* Yeast nitrogen base-amino acids/ammonium sulfate
