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Complete Minimal (CM) Dropout Medium

Per Liter:

  • 1.3 g dropout powder
  • 1.7 g YNB-AA/AS*
  • 5 g (NH4)2SO4
  • 20 g dextrose
Final concentration of nutrient in prepared media (µg/mL)
Adenine (hemisulfate salt) 40
L-aspartic acid 100
L-arginine (HCl) 20
L-glutamic acid (monosodium salt) 100
L-histidine 20
L-leucine 60
L-Lysine (mono-HCl) 30
L-methionine 20
L-phenylalanine 50
L-serine 375
L-threonine 200
L-tryptophan 40
L-tyrosine 30
L-valine 150
Uracil 20

* Yeast nitrogen base-amino acids/ammonium sulfate