DNase 1, RNase-Free, 2000U/mL, Pkg of 1, 0.5 mL #3D1401 Download SDS
Certificate of Analysis
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The CofA will be sent to you by email with 1 to 3 business days. Please refer to product packaging for Catalog Number and associated Lot Number and type exactly as shown.
- ADDITIONAL INFO
Additive Selection No Antibiotics Selection No LB Media Type No Organism No pH Range No Shipping Conditions Room Temperature Storage Conditions Refrigerate, 2-8°C.
Applications: 1. Degradation of DNA template in transcription reactions 2.Removal of contaminating genomic DNA from RNA samples 3. DNase I footprinting 4.Nick Translation
Properties and Usage: One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.
Reaction Conditions 1X DNase I Reaction Buffer Incubate at 37°C
1XDNase I Reaction Buffer: 10 mMTris-HCl, 2.5 mM MgCl2, 0.5 mM CaCl2, pH 7.6 @ 25°C
Heat Inactivation: 75°C for 10 min
Quality Control: Quality Control Assays:The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual.RNase Activity (1 Hour Digestion): The product is tested in a reaction containing a RNA substrate. After incubation for 1 hour there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.
EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation.