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DNase 1, RNase-Free, 2000U/mL

Recombinant Bovine pancreatic Deoxyribonuclease 1 produced in Pichiapastoris. DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.


Temperature sensitive items must be shipped overnight. 

Formulation

  • 10 mMTris-HCl, pH 7.6 @ 25°C
  • 2.5 mM MgCl
  • 20.5 mM CaCl
Fill Volume
0.5 mL
Packaging Size
1
Shipping Information
UPS Next Day Air
UPS Second Day Air
UPS Ground
Price
$81.64

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SDS

SDS

DNase 1, RNase-Free, 2000U/mL, Pkg of 1, 0.5 mL #3D1401 Download SDS

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CofA

Certificate of Analysis

Use fom below to request a Certificate of Analysis.

The CofA will be sent to you by email with 1 to 3 business days. Please refer to product packaging for Catalog Number and associated Lot Number and type exactly as shown.


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ADDITIONAL INFO

Additional Information

Additive Selection No
Antibiotics Selection No
LB Media Type No
Organism No
pH Range No
Shipping Conditions Room Temperature
Storage Conditions Refrigerate, 2-8°C.

Applications: 1. Degradation of DNA template in transcription reactions 2.Removal of contaminating genomic DNA from RNA samples 3. DNase I footprinting 4.Nick Translation

Properties and Usage: One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

Reaction Conditions 1X DNase I Reaction Buffer Incubate at 37°C

1XDNase I Reaction Buffer: 10 mMTris-HCl, 2.5 mM MgCl2, 0.5 mM CaCl2, pH 7.6 @ 25°C

Heat Inactivation: 75°C for 10 min

Quality Control: Quality Control Assays:The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual.RNase Activity (1 Hour Digestion): The product is tested in a reaction containing a RNA substrate. After incubation for 1 hour there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation.

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