The Benzonase® Nuclease is used for the removal of nucleic acid from protein samples. Benzonase nuclease can be used to degrade all forms of DNA and RNA while having no proteolytic activity. It can also be used to prepare proteins in microcalorimetric experiments.

Benzonase® is a genetically engineered endonuclease from Serratia marcescens. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is 8.0-9.2. It completely digests nucleic acids to 5'- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping.

Benzonase® Nuclease is supplied by Merck KGaA and its affiliates. Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Temperature sensitive items must be shipped overnight. 

Formulation

  • 20mM Tris HCl, pH 8.0
  • 2mM Magnesium Chloride
  • 20mM Sodium Chloride
  • 50% Glycerol
  • Benzonase Nuclease
3B5140
Benzonase Nuclease, 25U/µL, Pkg of 1, 100 µL Tube #3B5140
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Fill Volume: 100 mL
Packaging Size: 1 tubes
$74.00

SDS

Benzonase Nuclease, 25U/µL, Pkg of 1, 100 µL Tube #3B5140

Certificate of Analysis

Use the form below to request a Certificate of Analysis.

The CofA will be sent to you by email within 1 to 3 business days. Please refer to product packaging for the Catalog Number and associated Lot Number and type-in exactly as shown.

Request Certificate of Analysis

Additional Info

SKU AP0006
Additional Information
  • Benzonase can be inactivated using EDTA. Concentrations of greater than 1 mM EDTA will inhibit Benzonase activity. Irreversible inactivation can be accomplished with extreme conditions (100mM NaOH at 70°C for 30 minutes).

  • Benzonase is fairly stable if left out

Shipping Conditions On Ice
Storage Conditions Frozen