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Benzonase Nuclease, 25U/µL

The Benzonase® Nuclease is used for the removal of nucleic acid from protein samples. Benzonase nuclease can be used to degrade all forms of DNA and RNA while having no proteolytic activity. It can also be used to prepare proteins in microcalorimetric experiments.

BenzonaseÕŠëå is a genetically engineered endonuclease from Serratia marcescens. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is 8.0-9.2. It completely digests nucleic acids to 5Õ¢_ŠÎë_- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping.

Temperature sensitive items must be shipped overnight. 


  • 20 mM Tris HCl, pH 8.0
  • 2 mM MgCl2
  • 20 mM NaCl
Fill Volume
100 mL
Packaging Size
1 tubes
Shipping Information
UPS Next Day Air
UPS Second Day Air
UPS Ground

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Benzonase Nuclease, 25U/µL, Pkg of 1, 100 µL Tube #3B5140 Download SDS

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Certificate of Analysis

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The CofA will be sent to you by email with 1 to 3 business days. Please refer to product packaging for Catalog Number and associated Lot Number and type exactly as shown.

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Additional Information

Additive Selection No
Antibiotics Selection No
LB Media Type No
Organism No
pH Range No
Shipping Conditions No
Storage Conditions No

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