Protein extraction kit, contains:

• 20 mL 5X Aperto Cell Lysis Buffer
• 1.5 ml of each Solubilization Additives (5)
• User manual

Lysozyme(#3L2510 [view product]) and Benzonase (#3B5140) or DNase 1(#3D1401) are required for use with Aperto (not included).

Aperto is designed for use with E.coli for the lysis and extraction of proteins for analysis and further purification. It is a rapid, simple, and gentle method.

Aperto will solubilize proteins that tend to aggregate and remain in the insoluble fraction when using other commercially available products. It can be used exclusively or in conjunction with the included solubilization additives for the extraction of extremely difficult proteins. The solubilization additives stabilize proteins and prevent denaturation and subsequent aggregation and precipitation.