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COMPARISION OF APERTO TO VARIOUS POPULAR LYSIS BUFFERS

In the example below APERTO is compared to what we believe are the 3 most popular cell lysis products; represented by the letters U, C, and K. Aperto is represented by the letter A. Lysates from 2 different proteins are shown and the heterologous proteins are indicated with arrows

COMPARISION OF APERTO WITH A POPULAR LYSIS BUFFER ON DIFFICULT PROTEINS

Of the commercially available lysis buffers, one was more reliable than the others and was used in all subsequent comparisions. In the example shown below, cell lysates containing 6 different cloned genes in BL21(DE3) were lysed with the popular lysis product vs. Aperto. The panel to the right shows soluble protein. The panel to the left shows His-tag purified proteins from the same extracts. Proteins P1-P6 are described below

 

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