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  Home > Protein Extraction and Analysis > Protein Extraction and Purification >

Studier ZYM-5052 Medium Kit. 1L Kit. Cat.No. 3S2000
Studier ZYM-5052 Medium Kit. 1L Kit. Cat.No. 3S2000
Our Price: $45.95

Product Code: 3S2000

Description Supporting Documentation
Description: Studier Induction Media is based on Studier’s ZYM-5052 media and has been reformulated for ease of use. All of the ingredients are contained in 2 bottles instead of the usual 5. At the time of usage, aseptically add 60mL of Studier Salts Solution (Cat. No.3S2600 40mL included) to Studier ZYM-5052 Medium for Induction. (Cat. No.3S2200 940mL included). Media is stable for 2 months once the salt solution has been added. Note: this media contains glucose, lactose, and glycerol as carbon sources. No further additions are required.

Induction media works with all T7 regulated E. coli vectors in BL21 and derivative strains.


When glucose is present in the media the expression of the lactose permease is inhibited and lactose will not be transported into the cell. After approximately 12 hrs of growth the glucose will be consumed and the lactose permease will begin to express thereby transporting lactose into the cells.

In strains such as BL21 the T7 RNA polymerase has been inserted into the chromosome and is under control of the lacUV5 promoter and is therefore inducible with lactose. Genes cloned into vectors containing the T7 promoter (eg. pET 1 and Flexi®2 vectors) will not be expressed until all of the glucose in the media has been consumed. This is very useful for protein production and generally results in significantly higher protein yields than is obtain in conventional media.


1. pET vectors are available from EMD Millipore

2. Flexi® vectors are available from Promega.

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