View CartMy Account / Order StatusHelp
:searchbox-only>
 

*TIP omit words like "with" or "and" from your search for better results


Join our mailing list!


(Your shopping cart is empty)
  Home
About Us
 Contact Us
 Careers  
You are here: Home > Media for Nematode Research
We found 13 results matching your criteria. Narrow your search by selecting additional criteria below.
Nematode Growth Medium (NGM) for C. elegans

Caenorhabditits elegans, known as C. elegans, is a nematode (roundworm) approximately 1mm long that has a normal habitat in the soil. C. elegans is a model organism chosen by Syndey Brenner in the 1960's to study neural development in eukaryotes. Brenner wanted an organism with a simpler nervous system than that of Drosophilia (the fruit fly), the model organism of choice at that time. C. elegans was cheap, easy to grow in bulk, had far fewer neurons than Drosophila and offered the added advantage of being transparent so each stage of development could be easily observed. C. elegans has now been used to investigate the fundamental mechanisms of developmental biology, apoptosis, aging and RNA interference.

Strains of C. elegans are maintained in the lab on Nematode Growth Medium (NGM) according to the original recipe from Sydney Brenners lab. Small plates of 35mm diameter are useful for mating studies. Plates of 60mm diameter are used for general strain maintenance. Plates of 100mm diameter and larger (including 150mm and 245 x 245 Bioassay Q Trays) are used for growing larger quantities of worms, for example for mutant screens.

The standard recipe for NGM contains Tryptone, Sodium chloride (NaCl), Calcium Chloride (CaCl2), Magnesium Sulfate (MgSO4), Potassium Phosphate (KPO4), Cholesterol and Agar. A small amount of cholesterol (5g/ml) is added to the media to prevent the worms from going into an arrested developmental state known as dormant dauers. It is also understood that C. elegans require exogenous cholesterol for survival, although the effect of its depletion on nematode development is still considered controversial.

Nematode Growth Medium (NGM) (per Liter):
0.25% Tryptone (2.5g/L)

0.3% Sodium Chloride (NaCl) (3.0g/L)

1.5% Agar (15.0g/L)

1mM Calcium Chloride (CaCl2)

1mM Magnesium Sulfate (MgSO4)

25mM Potassium Phosphate (KPO4) pH 6.0

5g/mL Cholesterol



Sort By:
1
N0180
Nematode Growth Agar Plates with Tetracycline-12.5. 100mm, 20 plates, Sterile. Cat. No. N0180

Our Price: $62.92
N1105
Nematode Growth Media (NGM) Kit. 500g. Cat. No. N1105

Our Price: $87.80
N0197
Nematode Growth Media (NGM) with 100g/ml Ampicillin and 1mM IPTG. 60mm Plates, 20 plates. Sterile. Cat. No. N0197

Our Price: $52.34
N0188
Nematode Growth Media (NGM) with 2g/mL Uracil. 100mm, 20 plates. Sterile. Cat. No. N0188

Our Price: $53.35
N3060
Nematode Growth Media (NGM) with 25g/ml Kanamycin, 100g/ml Carbenicillin, 0.2mM Uracil, 10mM 3-AT and 0.01mM IPTG. 100mm, 20 plates per sleeve. Sterile. Cat. No. N3060

Our Price: $60.64
N3030
Nematode Growth Media (NGM) with 25g/ml Kanamycin, 100g/ml Carbenicillin, 0.2mM Uracil, 1mM 3-AT and 0.01mM IPTG. 100mm, 20 plates per sleeve. Sterile. Cat. No. N3030

Our Price: $60.64
N3040
Nematode Growth Media (NGM) with 25g/ml Kanamycin, 100g/ml Carbenicillin, 0.2mM Uracil, 3mM 3-AT and 0.01mM IPTG. 100mm, 20 plates per sleeve. Sterile. Cat. No. N3040

Our Price: $60.64
N3050
Nematode Growth Media (NGM) with 25g/ml Kanamycin, 100g/ml Carbenicillin, 0.2mM Uracil, 5mM 3-AT and 0.01mM IPTG. 100mm, 20 plates per sleeve.. Sterile. Cat. No. N3050

Our Price: $60.64
N3020
Nematode Growth Media (NGM) with 25g/ml Kanamycin, 100g/ml Carbenicillin, 0.2mM Uracil, and 0.01mM IPTG. 100mm, 20 plates per sleeve. Sterile. Cat.No. N3020

Our Price: $58.21
N0185
Nematode Growth Media (NGM) with 50g/ml Carbenicillin and 1mM IPTG. 100mm Plates, 20 plates. Sterile. Cat. No. N0185

Our Price: $65.06
N1098
Nematode Growth Media (NGM). 100mm, 20 plates. Sterile. Cat. No. N1098

Our Price: $40.83
N0199
Nematode Growth Media (NGM). 35mm, 10 plates per Sleeve. Sterile. Cat. No. N0199

Our Price: $58.97
N0179
Nematode Growth Media (NGM). 60mm, 20 Corning plates. Sterile. Cat. No. N0179

Our Price: $52.34
   
 
1
 

 About Us
 Contact Us
 Terms and Conditions
 Privacy Policy
 Send Us Feedback
 
Company Info| Product Index | Category Index | Help | Terms of Use
Copyright © www.teknova.com. All Rights Reserved. Order by Phone: 831-637-1100
Sell Online with Built with Volusion