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You are here: Home > Protein Extraction and Analysis > His-Tag Affinity Purification
We found 6 results matching your criteria. Narrow your search by selecting additional criteria below.

His-Tag Affinity Purification

Nickel Affinity Gel used for the purification of Histidine-tagged proteins. The hydrophilic linkage of the NTA chelate group to the agarose is highly selective for Histidine-tagged proteins. The matrix provides a simple and rapid one-step purification. Equilibration and Elution Buffers are provide. Both are designed to promote protein stabilization during purification and ensure his selectivity. The matrix can be used in either denaturing or non-denaturing conditions. The 5 mL Resin kit comes with 60mL Equilibration Buffer and 20mL Elution Buffer and the 25 mL Resin kit comes with 300mL Equilibration buffer and 100mL Elution buffer


  • >95% Pure Protein from Crude Lysate
  • Can be regenerated for re-use
  • Pre charged, ready to use matrices for any scale purification
BEAD (Geometry, Size) Spherical, Standard: ~ 50-170 Ám
CROSS-LINKED Yes
AGAROSE % 6% agarose
LIGAND Nitrilotriacetic acid (NTA)
BINDING CAPACITY >50 mg / ml gel*
ANTIMICROBIAL AGENT 30% Ethanol
STORAGE TEMPERATURE 4 - 8░
*Binding capacity will modify for each target protein.
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HIS-Capture Elution Buffer, 100mL. Cat.No. 3E3865
His-Capture Elution Buffer, 100mL. Cat.No. 3E3865

Our Price: $66.99
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His-Capture Equilibration Buffer, 300mL. Cat.No. 3E3850
His-Capture Equilibration Buffer, 300mL. Cat.No. 3E3850

Our Price: $90.09
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His-Tag Affinity Purification Kit . 25mL. Cat.No. 3N2320
His-Tag Affinity Purification Kit (25mL Resin with Buffers) Cat.No. 3N2320

Our Price: $294.52
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HIS-Capture Nickel Affinity Resin. 25mL. Cat.No. 3N2325
His-Capture Nickel Affinity Resin. 25mL. Cat.No. 3N2325

Our Price: $251.79
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His-Tag Affinity Purification Kit . 5mL. Cat.No. 3N2340
His-Tag Affinity Purification Kit (5mL Resin with Buffers) Cat.No. 3N2340

Our Price: $103.95
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HIS-Capture Nickel Affinity Resin. 5 mL. Cat.No. 3N2330
His-Capture Nickel Affinity Resin. 5 mL. Cat.No. 3N2330

Our Price: $251.79
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