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  Home > Liquid Culture Media > Bacterial Growth Media > MOPS EZ Rich Defined >

MOPS EZ Rich Defined Medium Kit. 5 Liter Cat.No. M2105
EZ Rich Defined Medium Kit. (5L) (Each) Cat. No. M2105
 
Our Price: $279.05

Product Code: M2105
Qty:

Description Supporting Documentation
 
Description:


Part # Description Amount
M2101 10X MOPS Mixture 100 mL
M2102 0.132 M K2HP04 10 mL
M2103 10X ACGU 100 mL
M2104 5X Supplement EZ 200 mL
W0225* Sterile H20* 580 mL
G0520 20% Glucose 10 mL
Total 1000 mL


Application: (Each kit makes 5 liters.) EZ Rich Medium is designed for the growth of E.coli and other enterobacteria. This formulation is a modification of Neidhardt supplemented MOPS defined media. Reference: Neidhardt, F. C., P. L. Bloch, and D. F. Smith. 1974. Culture medium for enterobacteria. J Bacteriol. 119:736-47
Shipping Conditions: On Ice/Ambient
Storage Conditions: M2101, M2103 and M2104 should be stored frozen. M2102 and G0520 should be stored at room temperature.

A 20 % solution of glucose (G0520) is supplied with this kit to be used as the carbon source
Additional concentrated sugar solutions are available in the Media Supplements section.

* Sterilized H2O is not included in the kit but may be ordered separately.

MOPS Media Formulations

M2101 MOPS Modified Rich Buffer
10X
Concentration
M2105/M2106
1X Concntration
MOPS (MW 209.3)
400mM
40mM
Tricine (MW 179.2)
40mM
4.0mM
Iron Sulfate Stock
0.1mM
0.01mM
Ammonium Chloride
95mM
9.5mM
Potassium Sulfate
2.76mM
0.276mM
Calcium Chloride
0.005mM
0.0005mM
Magnesium Chloride
5.25mM
0.525mM
Sodium Chloride
500mM
50mM
Ammonium Molybdate
3x10^-8 M
3x10^-9 M
Boric Acid
4x10^-6 M
4x10^-7 M
Cobalt Chloride
3x10^-7 M
3x10^-8 M
Cupric Sulfate
10^-7 M
10^-8 M
Manganese Chloride
8x10-7 M
8x10-8 M
Zinc Sulfate
10^-7 M
10^-8 M

M2102 Potassium Phosphate Dibsasic Solution
100X Concentration
M2105/M2106
1X Concntration
Potassium Phosphate Dibasic 132mM 1.32mM
M2104 5X Supplement EZ
5X concentration
M2105
1X concntration
L-Alanine
4.0mM
0.8mM
L-Arginine
26mM
5.2mM
L-Asparigine
2.0mM
0.4mM
L-Aspartic Acid, Potassium Salt
2.0mM
0.4mM
L-Glutamic Acid, Potassium Salt
3.3mM
0.66mM
L-Glutamine
3.0mM
0.6mM
L-Glycine
4.0mM
0.8mM
L-Histidine HCl H2O
1.0mM
0.2mM
L-Isoleucine
2.0mM
0.4mM
L-Proline
2.0mM
0.4mM
L-Serine
50mM
10mM
L-Threonine
2.0mM
0.4mM
L-Tryptophan
0.5mM
0.1mM
L-Valine
3.0mM
0.6mM
L-Leucine
4.0mM
0.8mM
L-Lysine
2.0mM
0.4mM
L-Methionine
1.0mM
0.2mM
L-Phenylalinine
2.0mM
0.4mM
L-Cysteine HCl
0.5mM
0.1mM
L-Tyrosine
1.0mM
0.2mM
Thiamine
0.05mM
0.01mM
Calcium Pantothenate
0.05mM
0.01mM
para-Amino Benzoic Acid
0.05mM
0.01mM
para-Hydroxy benzoic Acid
0.05mM
0.01mM
di Hydroxy Benzoic Acid
0.05mM
0.01mM

G0520 20% Glucose Solution
10X concentration
M2105/M2106 1X concentration
Glucose
20%
2.00%

M2103 ACGU Solution
10X
concentration
M2105
1X concntration
Potassium Hydroxide
15mM
1.5mM
Adenine
2.0mM
0.2mM
Cytosine
2.0mM
0.2mM
Uracil
2.0mM
0.2mM
Guanine
2.0mM
0.2mM


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