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  Home > Protein Extraction and Analysis > Protein Extraction and Purification >

DNAse 1 (RNAse-Free). 2,000 units/ml. 1000 units. 500 L Cat.No. 3D1401
DNAse 1 (RNAse-Free). 2000 units/mL.1000 units. Cat.No. 3D1401
 
Our Price: $76.23

Product Code: 3D1401
Qty:

Description Supporting Documentation
 
Description: Recombinant Bovine pancreatic Deoxyribonuclease 1 produced in Pichiapastoris. DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5-phosphorylated and 3-hydroxylated ends (1,2). DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.

Applications
  • Degradation of DNA template in transcription reactions
  • Removal of contaminating genomic DNA from RNA samples
  • DNase I footprinting
  • Nick Translation
Properties and Usage

One unit is defined as the amount of enzyme which will completely degrade 1 g of pBR322 DNA in 10 minutes at 37C in DNase I Reaction Buffer.

Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

Reaction Conditions 1X DNase I Reaction Buffer 
Incubate at 37C

1XDNase I Reaction Buffer:

 10 mMTris-HCl
2.5 mM MgCl2
0.5 mM CaCl2
pH 7.6 @ 25C

Storage Temperature
: -20C

Storage Conditions
: 10 mMTris-HCl
2 mM CaCl2
50% Glycerol
pH 7.6 @ 25C

Heat Inactivation
: 75C for 10 min

Quality Control
: Quality Control Assays:The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
RNase Activity (1 Hour Digestion):
The product is tested in a reaction containing a RNA substrate. After incubation for 1 hour there is no detectable degradation of the RNA substrate as determined by gel electrophoresis.

Notes
EDTA should be added to a final concentration of 5 mM to protect RNA from being degraded during enzyme inactivation

Technical Info - Comparision of Aperto to Various Popular Lysis Buffers
Technical Info - The Effect Of Solubilization Additives
Technical Poster - EXTRACTION AND SOLUBILIZATION OF DIFFICULT PROTEINS USING A NOVEL BUFFER SYSTEM


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