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Description: 10mM Tris-HCl, pH 8.0, 0.1mM EDTA, pH 8.0, sterile solution. TE buffer with reduced EDTA. Application: Tested for use in PCR, ligation, restriction digestion, DNA sequencing. Shipping Conditions: Ambient. Storage Conditions: Room Temperature.
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DNA Suspension Buffer
Overview: DNA suspension buffer is used to solubilize oligonucleotides, genomic DNA and plasmid DNA.
suspension buffer is also referred to as TElowE or 1TE0.1E. The purpose
of this buffer is to solubilize DNA or RNA, while protecting it from
degradation. Tris is used as a buffer rather than phosphate because
phosphate buffers are known to interfere with many enzymatic and
biochemical reactions. Also it is not possible to do an ethanol
precipitation of nucleic acids suspended in phosphate buffer.
EDTA chelates metal ions and it is added to sequester and bind divalent
cations, especially Ca2+ and Mg2+ ions which are required by nucleases,
the enzymes responsible for the degradation of DNA and RNA. EDTA is
added at 0.1mM, a low amount, so as to not interfere with downstream
reactions like PCR, restriction digests and ligations that typically
The solution is at pH 8.0 to reduce acid-catalyzed depurination of DNA.
RNA can be stored at a slightly lower pH of 7.5, because depurination
of RNA is base-catalyzed. However, it is safe to store both DNA and RNA
in this buffer at pH8.0.
Oligonucleotides, genomic DNA and plasmid DNA can be solubilized in
this buffer. Genomic and Plasmid DNA can be stored in this buffer at 4oC
for short-tem storage, but it is best to use -20oC or -80oC for
long-term storage. Repeated freeze-thaw cycles should be avoided to
minimize nucleic acid degradation.