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  Home > Protein Extraction and Analysis > Protein Extraction and Purification >

Benzonase« Nuclease (≥25U/ÁL). 100ÁL Tube. Cat.No. 3B5140
Benzonase® Nuclease  (≥25U/µL). 100µL Tube. Cat.No. 3B5140
 
Our Price: $59.22

Product Code: 3B5140
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Description Supporting Documentation
 


Description:
recombinant expressed in E. coli
assay ≥90% (SDS-PAGE)
form buffered aqueous glycerol solution
concentration 25 units/μL
storage temperature −20░C

Application: Used for the removal of nucleic acid from protein samples. Benzonase nuclease, or endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity. Benzonase nuclease can also be used to prepare proteins in microcalorimetric experiments Benzonase« is a genetically engineered endonuclease from Serratia marcescens. This endonuclease attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of operating conditions. The optimum pH for enzyme activity is 8.0-9.2. It completely digests nucleic acids to 5′- monophosphate terminated oligonucleotides 3 to 5 bases in length. This is ideal for removal of nucleic acids from recombinant proteins and for applications where complete digestion of nucleic acids is desirable. It also reduces viscosity in protein extracts and prevents cell clumping.

Unit Definition: One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 ░C (reaction volume 2.625 ml).
Storage Buffer: Solution in 50% glycerol containing 20 mM Tris HCl, pH 8.0, 2 mM MgCl2, and 20 mM NaCl.
Legal Information Benzonase« Nuclease is supplied by Merck KGaA and its affiliates. Benzonase is a registered trademark of Merck KGaA
Technical Info - Comparision of Aperto to Various Popular Lysis Buffers
Technical Info - The Effect Of Solubilization Additives
Technical Poster - EXTRACTION AND SOLUBILIZATION OF DIFFICULT PROTEINS USING A NOVEL BUFFER SYSTEM


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